Ph.D. (in Botany) (Title: Identification and profiling of miRNAs regulating triterpenoid biosynthetic pathway in Centella asiatica (L.) Urb)awarded on 17/08/2021
Masters in Plant Science Central University of Kerala, India.
Bachelor (in Botany), University of Calicut, India.
Ranjith, G. P., Jisha, S., Hemanthakumar, A. S., Saji, C. V., Shenoi, R. A., & Sabu, K. K. (2021). Impact of potential stimulants on asiaticoside and madecassoside levels and expression of triterpenoid- related genes in axenic shoot cultures of Centella asiatica (L.) Urb. Phytochemistry, 186, 112735- 112735. https://doi.org/10.1016/j.phytochem.2021.112735.
The findings provide molecular and biochemical evidence on silver nitrate acting as a metabolite stimulant, to enhance the formation of pharmaceutically important triterpenoids in axenic shoot culture of C. asiatica.
Ranjith, G. P., Satheeshan, J., & Sabu, K. K. (2020). Dataset on discovery of microRNAs in Centella asiatica (L.) Urb. Data in Brief, 33, 106451-106451. https://dx.doi.org/10.1016%2Fj.dib.2020.106451 The research work provides a brief description of the miRNA profile and its annotation of targets in C. asiatica, an important medicinal plant.
Jisha, S., Gouri, P. R., Hemantha Kumar, A. S., & Sabu, K. K. (2019). Expression profiling of selected microRNAs coupled with molecular and biochemical analyses of asiaticosides in Centella asiatica (L.) Urb. In vitro. International Journal of Pharmacy and Pharmaceutical Sciences, 11(5), 19–26. https://doi.org/10.22159/ijpps.2019v11i5.32097
The observations made through this work establishes direct correlation between oxidative stress and transcript/phytochemical estimation of asiaticoside content under in vitro conditions in C. asiatica.
Jisha, S., Gouri, P. R., Anith, K. N., & Sabu, K. K. (2018). Piriformospora indica cell wall extract as the best elicitor for asiaticoside production in Centella asiatica (L.) Urban, evidenced by morphological, physiological and molecular analyses. Plant Physiology and Biochemistry, 125, 106-115. https://doi.org/10.1016/j.plaphy.2018.01.021
The study demonstrates the potential use of the fungus, Piriformospora indica as a means to enhance secondary metabolite production in plants.
Ranjith, G. P., Jisha, Sabu, K. K. Unraveling MicroRNA Mediated Gene Regulation in Centella Asiatica (L.) Urb. by High-Throughput Sequencing based Small-RNA Profiling (Pre-print) https://doi.org/10.21203/rs.3.rs-995211/v1
The study represents the large-scale identification of microRNAs from C. asiatica using high-throughput sequencing and contributes to the base for the up-coming studies on miRNA-mediated gene regulation of plant secondary metabolite pathways in particular.
Jisha S, Aiswarya S, Gouri PR, Manikantan K & Sabu KK. Impact of mannitol and poly ethylene glycol 6000 induced water deficit on plant biomass and major secondary metabolites in Centella asiatica (L.) Urb. in vitro. Indian Journal of Experimental Biology Vol. 60, March 2022, pp. 184-191
Participation in Conferences and Workshop
“Metabolite stimulant responsive gene expression regulation of microRNAs in axenic shoot cultures of Centella asiatica (L.) Urb.” International Webinar on Advances in Genomics & Gene Technology, Inter University Centre for Genomics & Gene Technology Department of Biotechnology, University of Kerala, India 2021. — Best paper presentation award
“Salicylic acid responsive transcriptional regulation of miRNAs in in vitro cultures of Centella asiatica (L.) Urb.” KSCSTE-JNTBGRI webinar series, Recent Trends in Plant Science Research at JNTBGRI, 2020.
“Elicitor Induced Modulation of Asiaticoside and Expression of Biosynthetic Pathway Genes in Centella asiatica (L.) Urb.” International Conference on ‘Innovative and Emerging Trends in Botany’ (ICIETB) Dept. Of Botany, Alagappa University, Tamil Nadu, India, 2019.
“Identification of conserved and novel microRNAs involved in asiaticoside biosynthesis in Centella asiatica” Nextgen Genomics, Biology, Bioinformatics and Technologies (NGBT) Conference, Mumbai, India, 2019.
“Identification of conserved microRNAs in Centella asiatica (L.) Urb. and computational prediction of their targets involved in asiaticoside biosynthesis”. International Conference on Emerging Areas in Biotechnology for Human Welfare Bioentrepreneurship, B.S. Abdur Rahman Crescent Institute of Science & Technology, Chennai, India, 2018. — Best poster presentation award.
“Decoding the non-coding”- Work shop on functional analysis of miRNAs, conducted by Department Biochemistry and Molecular biology, Central University of Kerala, India from February 15 to 20, 2016.
“Next-Generation RNA Sequence Data Analysis”, conducted by JNTBGRI and sponsored by DBT (Dept. of Biotechnology, Govt. of India) from March 6-10, 2017.
Fellowships and awards
Research Fellowship from Kerala State Council for Science, Technology and Environment (KSCSTE) Research Fellowship, 2015.
Recipient of Council for Scientific and Industrial Research (CSIR) NET (National Eligibility Test) in Life Sciences (2015, Indian National Level award).
Qualified GATE (Graduate Aptitude Test in Engineering, 2014) in Life Sciences (National Level Exam).
Merit Scholarship (2012-2014, University Level Scholarship during Master’s Degree).
Get in touch with Us!
St. Thomas' College (Autonomous) Thrissur - 680 001, Kerala, India